Molecular Imaging Webinar Series
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"Illumination Sources for Fluorescence Applications"
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Abstract:
Fluorescent molecules are beacons that can absorb photons of light of a higher energy (short wavelength) and then emit photons at a longer wavelength with less energy. These fluorescent molecules have been demonstrated to be useful for a wide variety of applications in biological research. For example, in-vitro assays such as microscopy and cell sorting (FACS) have used both single as well as multiplex labeling approaches to address complex questions. More recently, fluorescent molecules have been gaining utility as labels for antibodies to generate probes for detection of proteins in Western blotting applications.
Whatever the application, fluorescent molecules require a source of excitation. This can be delivered by three main types of illumination sources: lasers, broad spectrum filtered light and, more recently, light emitting diodes (LED’s). This webinar will present some of the pros and cons for each type of illumination source with an emphasis on their use for detection of labels in fluorescence Western blotting applications. |
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